ÿþ<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> <html xmlns="http://www.w3.org/1999/xhtml"> <head> <title>Gotu Kola Extract,Centella Asiatica PE-Asiaticoside,Madecassoside,Madecassic acid,Asiatic acid,Centella Asiatica Extract</title> <meta name="Description" content="Gotu Kola Extract(Centella Asiatica PE),which contains 10%-90% Asiaticoside,Asiatic acid,Madecassoside,Madecassic Acid,are used for Cosmetics for many years.Asiaticoside Protecting skin tissue,skin tuberculosis and lepra.Centella Asiatica Extract"> <meta name="Keywords" content="Gotu Kola Extract,Centella Asiatica PE,Asiaticoside,Madecassoside,Madecassic acid,Asiatic acid,Centella Asiatica Extract,Asiaticoside manufacture,Gotu Kola Extract manufacture"> <meta name="robots" content="index,follow,all"> <meta http-equiv="Content-Type" content="text/html; charset=gb2312" /> <link href="css/style01.css" rel="stylesheet" type="text/css" /> </head> <body><h1 align='center'><P><A href="http://www.plamed.cn"></A></P> <P><FONT color=#0000ff size=6></P><FONT color=#110700><SPAN style="FONT-FAMILY: 'Times New Roman'; FONT-SIZE: 10.5pt; mso-bidi-font-size: 12.0pt; mso-font-kerning: 1.0pt; mso-fareast-font-family: ; mso-fareast-language: ZH-CN; mso-ansi-language: EN-US; mso-bidi-language: AR-SA" lang=EN-US></SPAN></FONT></FONT> <P></P><FONT color=#110700 size=3> <H3><BR><FONT size=6 face="Arial Black"></FONT>&nbsp;<A href="http://www.plamed.cn/" target=_blank><IMG border=0 src="/UploadFiles/2011/10/201110110839316417.jpg"></A></H3> <H3><FONT size=6 face="Arial Black">Gotu Kola Extract</FONT></H3> <H3></FONT><FONT color=#110700 size=3>Asiaticoside Introduction,Madecassoside Report&nbsp;</FONT></H3></h1> <div class='btitle'><h2>What is Gotu Kola Extract( Centella asiatica PE )</h2><div class='content'><a href='http://www.asiaticoside.com/images/Gotu-Kola.jpg' target='_blank'><img src='http://www.asiaticoside.com/images/Gotu-Kola.jpg' alt=asiaticoside' /></a><span class='hj'><P><FONT size=2><STRONG>Gotu Kola Extract </STRONG>(Centella asiatica PE) is extracted from the all grass of Dicotyledons umbrelliferae Gotu Kola (Centella asiatica l.) Urban. Gotu kola is a medicinal plant that has been used since prehistoric times.Centella asiatica is used in folk medicine to treat a wide range of medical conditions. This herb has been subjected to quite extensive experimental and clinical investigations.<BR></FONT><FONT size=2><STRONG>Gotu Kola Extract</STRONG>&nbsp;(Centella asiatica Extract) has a positive effect in the treatment of venous insufficiency and striae gravidarum. Centella asiatica also appears to be effective in the treatment of wound healing disturbances. <BR></FONT><FONT size=2><STRONG>[solubility]:</STRONG>Insoluble in water, but soluble in alcohol and pyridine<BR><STRONG>[Extracted source]:</STRONG>the all grass of Dicotyledons umbrelliferae Gotu Kola (Centella asiatica l.) Urban.<BR><STRONG>[Chemical composition]:Aticoside, Madecassoside, Asiatic acid and Madecassic acid<BR></STRONG><STRONG>[Characters]:</STRONG>Yellowish brown to white fine powder<BR><STRONG>[Market specifications]:Aticoside10%~95%;Madecassoside10%~95%;Asiatic acid 60%,95%;Madecassic acid 50%,95%<BR>[Dosage]:</STRONG>The general dose for Gotu Kola Extract is 3 times one day, each time 20-40 mg standard contains 40% Aticoside. Containing more than 70% Aticoside and Madecassoside is 60mg every time ,1-2times one day. <BR><STRONG>[Application dosage form]:</STRONG>Suppositories, lotion, injection, tablet, capsule etc.<BR><STRONG>[Storage conditions]:</STRONG>Cool and dry, avoid light<STRONG><STRONG><STRONG><STRONG><STRONG><STRONG><STRONG><STRONG></FONT></P></STRONG></STRONG></STRONG></STRONG></STRONG></STRONG></STRONG></STRONG></span></div></div> <div class='btitle'><h2>Indications and Uses of Gotu Kola Extract( Centella asiatica PE )</h2><div class='content'><a href='http://www.Asiaticoside.com/images/Gotu-Kola-cosmetic.jpg' target='_blank'><img src='http://www.Asiaticoside.com/images/Gotu-Kola-Extracts.jpg' alt=Gotu-Kola-cosmetic.jpg' /></a><span class='hj'><P><STRONG>Indications and Uses of Gotu Kola Extract </STRONG><FONT size=2>(Centella asiatica Extract)</FONT><BR><BR><STRONG>Indications and Uses<BR></STRONG>(1)Regulating immunity and improving blood circulation<BR>(2)Lowering cholesterinand and getting rid of excess fat.<BR>(3)Protecting skin tissue, promoting wound healing, treating skin ulcer, such as intractable trauma,skin tuberculosis and lepra.<BR>(4)Owning good therapeutic effect on venous infufficiency and varicose vein<BR>(5)Improving sleep quality and enhancing memory ability .<BR>(6)Alleviating arthrodynia and scleriasis </P> <DIV align=left><B>Application:</B></DIV> <DIV align=left>(1)Applied in phamaceutical field, it is used as phamaceutical raw material to treat injuries from fall, infectious hepatitis and epidemic cerebrospinal meningitis.</DIV> <DIV align=left>(2)Applied in healthcare field, it is used in the health products to nourish brain, protect liver and improve sleep quality.</DIV> <DIV align=left>(3)Applied in comestics, it is used to promote skin metabolism and make skin more tender and firm</DIV></span></div></div> <div class='menu'> <div class='orders'><h2><a href='articles.asp'>Article Directory</a></h2></div><ul><li><strong><a href='#3' >The identification of the Asiaticoside</a></strong></li><li><strong><a href='#16' >The identification of Madecassoside</a></strong></li><li><strong><a href='#20' >HPLC analysis method of Gotu Kola Extract ( Centella asiatica PE )</a></strong></li><li><strong><a href='#24' >The extract method of asiaticoside and madecassoside</a></strong></li><li><strong><a href='#25' >The HPLC method of asiaticoside in Centella asiatica extract</a></strong></li><li><strong><a target='_blank' href='Asiatic-acid.html' >The identification of Asiatic acid </a></strong></li><li><strong><a target='_blank' href='Madecassic-acid.html' >The identification of Madecassic acid </a></strong></li><li><strong><a target='_blank' href='Gotu-Kola-Extract.html' >Market specifications of Gotu Kola Extract ( Centella asiatica PE )</a></strong></li><li><strong><a target='_blank' href='Production-of-asiaticoside.html' >Production of asiaticoside and madecassoside</a></strong></li><li><strong><a target='_blank' href='articles.asp' >More>></a></strong></li></ul></div><div class='cls'></div><div class='mlist'><h2 align='center'><a name='3' id='3' ></a>The identification of the Asiaticoside</h2><p><P> <TABLE cellSpacing=1 width="95%" border=0> <TBODY> <TR> <TD class=style3 vAlign=center align=middle width="95%" colSpan=3 height=1> <H4><FONT color=#002f64>Asiaticoside </FONT></H4></TD></TR> <TR bgColor=#fcf7f2> <TD class=style2 align=middle width="100%" colSpan=3 height=21><B>Identification</B></TD></TR> <TR> <TD class=style2 align=right width="30%" height=21>Name</TD> <TD class=style2 width="2%" height=21></TD> <TD class=style2 width="68%" height=21>Asiaticoside</TD></TR> <TR> <TD class=style2 align=right width="30%" height=21>Synonyms</TD> <TD class=style2 width="2%" height=21></TD> <TD class=style2 width="68%" height=21> <P style="LINE-HEIGHT: 150%" align=left><SPAN lang=EN-US style="FONT-SIZE: 10.5pt; LINE-HEIGHT: 150%; FONT-FAMILY: Verdana">[6-[[3,4-Dihydroxy-6-(hydroxymethyl)-5-(3,4,5-trihydroxy-6-methyl-oxan-2-yl)oxy-oxan-2-yl]oxymethyl]-3,4,5-trihydroxy-oxan-2-yl] (1S,2R,4aS,6aS,6bR,9S,10R,11R,12aS,14bR)-10,11-dihydroxy-9-(hydroxymethyl)-1,2,<?xml:namespace prefix = st1 ns = "urn:schemas-microsoft-com:office:smarttags" /><st1:chmetcnv UnitName="a" SourceValue="6" HasSpace="False" Negative="False" NumberType="1" TCSC="0" w:st="on">6a</st1:chmetcnv>,6b,9,<st1:chmetcnv UnitName="a" SourceValue="12" HasSpace="False" Negative="False" NumberType="1" TCSC="0" w:st="on">12a</st1:chmetcnv>-hexamethyl-2,3,4,5,6,<st1:chmetcnv UnitName="a" SourceValue="6" HasSpace="False" Negative="False" NumberType="1" TCSC="0" w:st="on">6a</st1:chmetcnv>,7,8,<st1:chmetcnv UnitName="a" SourceValue="8" HasSpace="False" Negative="False" NumberType="1" TCSC="0" w:st="on">8a</st1:chmetcnv>,10,11,12,13,14b-tetradecahydro-1H-picene<st1:chmetcnv UnitName="a" SourceValue="4" HasSpace="False" Negative="True" NumberType="1" TCSC="0" w:st="on">-4a</st1:chmetcnv>-carboxylate<?xml:namespace prefix = o ns = "urn:schemas-microsoft-com:office:office" /><o:p></o:p></SPAN></P></TD></TR> <TR> <TD class=style2 align=right width="30%" height=21>Molecular Structure</TD> <TD class=style2 width="2%" height=21></TD> <TD class=style2 onmouseover=showIt() onmouseout=hideIt() width="68%" height=21><IMG src="/UploadFiles/2010/12/201012021534052527.bmp" border=0></TD></TR> <TR> <TD class=style2 align=right width="30%" height=21>Molecular Formula</TD> <TD class=style2 width="2%" height=21></TD> <TD class=style2 width="68%" height=21>C<SUB>48</SUB>H<SUB>78</SUB>O<SUB>19</SUB></TD></TR> <TR> <TD class=style2 align=right width="30%" height=21>Molecular Weight</TD> <TD class=style2 width="2%" height=21></TD> <TD class=style2 width="68%" height=21>959.12</TD></TR> <TR> <TD class=style2 align=right width="30%" height=21>CAS Registry Number</TD> <TD class=style2 width="2%" height=21></TD> <TD class=style2 width="68%" height=21>16830-15-2</TD></TR> <TR> <TD class=style2 align=right width="30%" height=21>EINECS</TD> <TD class=style2 width="2%" height=21></TD> <TD class=style2 width="68%" height=21>240-851-7</TD></TR></TBODY></TABLE></P> <HR></p><div class='jiange'></div><h2 align='center'><a name='16' id='16' ></a>The identification of Madecassoside</h2><p><TABLE cellSpacing=1 width="95%" border=0> <TBODY> <TR> <TD class=style3 vAlign=center align=middle width="95%" colSpan=3 height=1> <H4><FONT color=#002f64>Madecassoside </FONT></H4></TD></TR> <TR bgColor=#fcf7f2> <TD class=style2 align=middle width="100%" colSpan=3 height=21><B>Identification</B></TD></TR> <TR> <TD class=style2 align=right width="30%" height=21>Name</TD> <TD class=style2 width="2%" height=21></TD> <TD class=style2 width="68%" height=21>Madecassoside</TD></TR> <TR> <TD class=style2 align=right width="30%" height=21>Synonyms</TD> <TD class=style2 width="2%" height=21></TD> <TD class=style2 width="68%" height=21>O-6-Deoxy-alpha-L-mannopyranosyl-(1.4)-O-beta-D-glucopyranosyl-(1.6)-beta-D-glucopyranosyl (2alpha,3beta,4alpha,6beta)-2,3,6,23-tetrahydroxyurs-12-en-28-oate</TD></TR> <TR> <TD class=style2 align=right width="30%" height=21>Molecular Structure</TD> <TD class=style2 width="2%" height=21></TD> <TD class=style2 onmouseover=showIt() onmouseout=hideIt() width="68%" height=21><IMG src="/UploadFiles/2010/12/201012021536214086.bmp" border=0></TD></TR> <TR> <TD class=style2 align=right width="30%" height=21>Molecular Formula</TD> <TD class=style2 width="2%" height=21></TD> <TD class=style2 width="68%" height=21>C<SUB>48</SUB>H<SUB>78</SUB>O<SUB>20</SUB></TD></TR> <TR> <TD class=style2 align=right width="30%" height=21>Molecular Weight</TD> <TD class=style2 width="2%" height=21></TD> <TD class=style2 width="68%" height=21>975.12</TD></TR> <TR> <TD class=style2 align=right width="30%" height=21>CAS Registry Number</TD> <TD class=style2 width="2%" height=21></TD> <TD class=style2 width="68%" height=21>34540-22-2</TD></TR> <TR> <TD class=style2 align=right width="30%" height=21>EINECS</TD> <TD class=style2 width="2%" height=21></TD> <TD class=style2 width="68%" height=21>252-076-1</TD></TR></TBODY></TABLE> <HR></p><div class='jiange'></div><h2 align='center'><a name='20' id='20' ></a>HPLC analysis method of Gotu Kola Extract ( Centella asiatica PE )</h2><p><P style="LINE-HEIGHT: 200%" align=left><SPAN lang=EN-US style="FONT-SIZE: 10.5pt; LINE-HEIGHT: 200%; FONT-FAMILY: Verdana">An HPLC method, with the addition of Beta-cyclodextrin in the mobile phase, was established for the determination of asiaticoside, madecassoside and asiaticoside B in the extract of Centella triterpenes. <BR><BR>A C<SUB>18</SUB> column was used with the mobile phase of acetonitrile-4 mmol/L of Beta-cyclodextrin solution (25:75) at the detection wavelength of 204 nm. <?xml:namespace prefix = o ns = "urn:schemas-microsoft-com:office:office" /><o:p></o:p></SPAN></P> <P style="LINE-HEIGHT: 200%" align=left><SPAN lang=EN-US style="FONT-SIZE: 10.5pt; LINE-HEIGHT: 200%; FONT-FAMILY: Verdana">The calibration curves of <A href="http://www.asiaticoside.com/Asiaticoside.html" target=_blank><FONT color=#0000ff>asiaticoside</FONT></A>, <A href="http://www.asiaticoside.com/Madecassoside.html" target=_blank><FONT color=#0000ff>madecassoside</FONT></A> and asiaticoside B were linear in the range of 2 - 20 ug.The average recoveries were 99.25%, 98.41% and 100.15%, with the RSDs of 1.12%, 1.21% and 1.20%, respectively.<o:p></o:p></SPAN></P> <P align=left>&nbsp;</P></p><div class='jiange'></div><h2 align='center'><a name='24' id='24' ></a>The extract method of asiaticoside and madecassoside</h2><p><P align=left><FONT size=3>The extract method of asiaticoside and madecassoside from Centella asiatica.<BR><BR>This article introduces how to extract asiaticoside and madecassoside from Centella asiatica,and asiaticoside and madecassoside obtained is water-soluble.<BR><BR>In the isolating way an aqueous alcohol containing Centella asiatica is subjected to cold-precipitation to get an extract. <BR><BR>The Centella asiatica PE in the aqueous alcohol is treated with a halogenide solvent for layer separation, and then the aqueous layer is extracted with a higher alcohol.<BR><BR>The Centella asiatica PE in the alcohol layer is washed first with sodium hydroxide and then with water.<BR><BR>An HPLC method of asiaticoside and madecassoside : </FONT><A href="http://www.asiaticoside.com/Asiaticoside-analysis.html"><FONT size=3>http://www.asiaticoside.com/Asiaticoside-analysis.html</FONT></A><BR><FONT size=3>After being concentrated, Centella asiatica PE is crystallized with ethyl acetate to get crystals and the crystals are washed. <BR><BR>If you'd like to view the full article, please visit:<BR></FONT><A href="http://www.asiaticoside.com/articles/isolating-method-of-asiaticoside-and-madecassoside.pdf"><FONT size=3>http://www.asiaticoside.com/articles/isolating-method-of-asiaticoside-and-madecassoside.pdf</FONT></A></P> <P align=left><FONT size=3>If you'd like to view more article about Centella asiatica PE, please visit: <BR></FONT><A href="http://www.asiaticoside.com"><FONT size=3>http://www.asiaticoside.com</FONT></A></P></p><div class='jiange'></div><h2 align='center'><a name='25' id='25' ></a>The HPLC method of asiaticoside in Centella asiatica extract</h2><p><P class=MsoNormal style="MARGIN: 0cm 0cm 0pt"><SPAN lang=EN-US><?xml:namespace prefix = o ns = "urn:schemas-microsoft-com:office:office" /><o:p><FONT face="Times New Roman" size=3>&nbsp;</FONT></o:p></SPAN></P> <DIV align=right> <TABLE class=MsoTableGrid style="BORDER-RIGHT: medium none; BORDER-TOP: medium none; MARGIN: auto 6.75pt; BORDER-LEFT: medium none; BORDER-BOTTOM: medium none; BORDER-COLLAPSE: collapse; mso-yfti-tbllook: 480; mso-padding-alt: 0cm 5.4pt 0cm 5.4pt; mso-table-lspace: 9.0pt; mso-table-rspace: 9.0pt; mso-table-anchor-vertical: paragraph; mso-table-anchor-horizontal: page; mso-table-left: right; mso-table-top: 39.1pt; mso-border-alt: solid white .5pt; mso-border-insideh: .5pt solid white; mso-border-insidev: .5pt solid white" cellSpacing=0 cellPadding=0 align=right border=1> <TBODY> <TR style="HEIGHT: 172.25pt; mso-yfti-irow: 0; mso-yfti-firstrow: yes; mso-yfti-lastrow: yes"> <TD style="BORDER-RIGHT: white 1pt solid; PADDING-RIGHT: 5.4pt; BORDER-TOP: white 1pt solid; PADDING-LEFT: 5.4pt; PADDING-BOTTOM: 0cm; BORDER-LEFT: white 1pt solid; WIDTH: 157.7pt; PADDING-TOP: 0cm; BORDER-BOTTOM: white 1pt solid; HEIGHT: 172.25pt; BACKGROUND-COLOR: transparent; mso-border-alt: solid white .5pt" vAlign=top width=210> <P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 150%; mso-element: frame; mso-element-frame-hspace: 9.0pt; mso-element-wrap: around; mso-element-anchor-vertical: paragraph; mso-element-anchor-horizontal: page; mso-element-top: 39.1pt; mso-height-rule: exactly; mso-element-left: right"><SPAN lang=EN-US style="FONT-FAMILY: Verdana; mso-bidi-font-family: &#718;&#805;"><?xml:namespace prefix = v ns = "urn:schemas-microsoft-com:vml" /><v:shapetype id=_x0000_t75 stroked="f" filled="f" coordsize="21600,21600" o:spt="75" o:preferrelative="t" path="m@4@5l@4@11@9@11@9@5xe"><v:stroke joinstyle="miter"></v:stroke><v:formulas><v:f eqn="if lineDrawn pixelLineWidth 0"></v:f><v:f eqn="sum @0 1 0"></v:f><v:f eqn="sum 0 0 @1"></v:f><v:f eqn="prod @2 1 2"></v:f><v:f eqn="prod @3 21600 pixelWidth"></v:f><v:f eqn="prod @3 21600 pixelHeight"></v:f><v:f eqn="sum @0 0 1"></v:f><v:f eqn="prod @6 1 2"></v:f><v:f eqn="prod @7 21600 pixelWidth"></v:f><v:f eqn="sum @8 21600 0"></v:f><v:f eqn="prod @7 21600 pixelHeight"></v:f><v:f eqn="sum @10 21600 0"></v:f></v:formulas><v:path o:extrusionok="f" gradientshapeok="t" o:connecttype="rect"></v:path><o:lock v:ext="edit" aspectratio="t"></o:lock></v:shapetype><v:shape id=_x0000_i1025 style="WIDTH: 143.25pt; HEIGHT: 159.75pt" type="#_x0000_t75"><v:imagedata src="file:///C:\DOCUME~1\ADMINI~1\LOCALS~1\Temp\msohtml1\01\clip_image001.jpg" o:title="HPLC"><FONT face="Times New Roman" size=3><IMG src="http://www.asiaticoside.com/photo/HPLC.bmp" border=0></FONT></v:imagedata></v:shape><o:p></o:p></SPAN></P></TD></TR></TBODY></TABLE></DIV> <P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 150%" align=left><SPAN lang=EN-US style="FONT-FAMILY: Verdana; mso-bidi-font-family: &#718;&#805;"><FONT size=2>The simple and high performance HPLC method of asiaticoside in Centella asiatica extract. <o:p></o:p></FONT></SPAN></P> <P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 150%" align=left><SPAN lang=EN-US style="FONT-FAMILY: Verdana; mso-bidi-font-family: &#718;&#805;"><o:p><FONT size=2>&nbsp;</FONT></o:p></SPAN></P> <P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 150%" align=left><SPAN lang=EN-US style="FONT-FAMILY: Verdana; mso-bidi-font-family: &#718;&#805;"><FONT size=2>This article introduces a simple, sensitive high performance liquid chromatographic method of asiaticoside in </FONT><A href="http://www.asiaticoside.com/Asiaticoside.html" target=_blank><FONT color=#800080 size=2>Centella asiatica extract</FONT></A><FONT size=2>. <o:p></o:p></FONT></SPAN></P> <P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 150%" align=left><SPAN lang=EN-US style="FONT-FAMILY: Verdana; mso-bidi-font-family: &#718;&#805;"><o:p><FONT size=2>&nbsp;</FONT></o:p></SPAN></P> <P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 150%" align=left><SPAN lang=EN-US style="FONT-FAMILY: Verdana; mso-bidi-font-family: &#718;&#805;"><FONT target="_blank"><FONT size=2>About the extract method of Centella asiatica extract, please visit:<BR><BR></FONT><A title="The extract method of asiaticoside and madecassoside" href="http://www.asiaticoside.com/asiaticoside-madecassoside.html" target=_blank><FONT color=#0000ff size=2>The extract method of asiaticoside and madecassoside </FONT></A><BR><BR></FONT></P><o:p></o:p></SPAN> <P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 150%" align=left><SPAN lang=EN-US style="FONT-FAMILY: Verdana; mso-bidi-font-family: &#718;&#805;"><FONT size=2>The asiaticoside yielded 1.7mg/100 mg of the test </FONT><A href="http://www.asiaticoside.com/Asiaticoside.html" target=_blank><FONT color=#800080 size=2>Centella asiatica extract</FONT></A><FONT size=2> . <o:p></o:p></FONT></SPAN></P> <P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 150%" align=left><SPAN lang=EN-US style="FONT-FAMILY: Verdana; mso-bidi-font-family: &#718;&#805;"><o:p><FONT size=2>&nbsp;</FONT></o:p></SPAN></P> <P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 150%" align=left><SPAN lang=EN-US style="FONT-FAMILY: Verdana; mso-bidi-font-family: &#718;&#805;"><FONT size=2>Calibration graph of </FONT><A href="http://www.asiaticoside.com/Asiaticoside.html" target=_blank><FONT color=#800080 size=2>asiaticoside</FONT></A><FONT size=2> showed good linearity in the concentration range of (32 - 1027 ug/mL), with a correlation coefficient of 0.999. <o:p></o:p></FONT></SPAN></P> <P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 150%"><SPAN lang=EN-US style="FONT-FAMILY: Verdana; mso-bidi-font-family: &#718;&#805;"><o:p><FONT size=2>&nbsp;</FONT></o:p></SPAN></P> <P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 150%" align=left><SPAN lang=EN-US style="FONT-FAMILY: Verdana; mso-bidi-font-family: &#718;&#805;"><FONT size=2>The average recovery for </FONT><A href="http://www.asiaticoside.com/Asiaticoside.html" target=_blank><FONT color=#800080 size=2>asiaticoside</FONT></A><FONT size=2> is 97.5 %. <BR><BR>The proposed method is reproducible and statistically validated. <o:p></o:p></FONT></SPAN></P> <P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 150%" align=left><SPAN lang=EN-US style="FONT-FAMILY: Verdana; mso-bidi-font-family: &#718;&#805;"><o:p><FONT size=2>&nbsp;</FONT></o:p></SPAN></P> <P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 150%" align=left><SPAN lang=EN-US style="FONT-FAMILY: Verdana; mso-bidi-font-family: &#718;&#805;"><FONT size=2>The system was successfully used to investigate the presence of the </FONT><A href="http://www.asiaticoside.com/Asiaticoside.html" target=_blank><FONT color=#800080 size=2>asiaticoside</FONT></A><FONT size=2> in Centella asiatica plant parts.<BR style="mso-special-character: line-break"><BR style="mso-special-character: line-break"></FONT></P><o:p></o:p></SPAN> <P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 150%" align=left><SPAN lang=EN-US style="FONT-FAMILY: Verdana; mso-bidi-font-family: &#718;&#805;"><FONT size=2>If you'd like to view the full article, please visit: <BR><BR><o:p></o:p></FONT></SPAN></P> <P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 150%" align=left target="_blank"><SPAN lang=EN-US style="FONT-FAMILY: Verdana; mso-bidi-font-family: &#718;&#805;"><A href="http://www.asiaticoside.com/articles/The-HPLC-method-of-asiaticoside.pdf"><FONT color=#0000ff size=2>http://www.asiaticoside.com/articles/The-HPLC-method-of-asiaticoside.pdf</FONT></A></SPAN><SPAN lang=EN-US style="LINE-HEIGHT: 150%; FONT-FAMILY: Verdana; mso-bidi-font-family: &#718;&#805;; mso-bidi-font-size: 10.5pt"><BR style="mso-special-character: line-break"><BR style="mso-special-character: line-break"></P></SPAN><SPAN lang=EN-US style="FONT-FAMILY: Verdana; mso-bidi-font-family: &#718;&#805;"><o:p></o:p></SPAN> <P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 150%" align=left><SPAN lang=EN-US style="FONT-FAMILY: Verdana; mso-bidi-font-family: &#718;&#805;"><FONT size=2>If you'd like to view more article about Centella asiatica PE, please visit: </FONT><A href="http://www.asiaticoside.com/" target=_blank><FONT color=#800080 size=2>http://www.asiaticoside.com</FONT></A></SPAN><SPAN class=MsoHyperlink><SPAN lang=EN-US><o:p></o:p></SPAN></SPAN></P> <P class=MsoNormal style="MARGIN: 0cm 0cm 0pt; 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